Bca working reagent reagent a mixed with reagent b is stable for one day. Pierce bca protein assay kit reagent a was combined with pierce bca protein assay kit reagent b in a fifty to one ratio 50a. Pierce bca reagents pierce bca protein assay reagent b. Bca reagents a and b, compatibility reagent, reconstitution buffer, and albumin standard 2mgml.
The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Procedure in the standard assay, 20 parts of the bca working reagent are then mixed with 1 part of a protein sample. Quick start bradford protein assay instruction manual. Protocol for bca protein assay using a nanodrop 20002000c. Pierce microplate bca protein assay kit reducing agent compatible, sufficient reagents for. Description the pierce rapid gold bca protein assay kit is a fast, twocomponent, highprecision, detergentcompatible assay optimized to determine total protein concentration. Thermo scientific pierce bca protein assay kit bca protein. Compared to most dyebinding methods, the bca assay is affected much less by protein compositional differences, providing greater proteintoprotein uniformity. Using the matrix platemate 2x2 to automate the bca protein. Pierce rapid gold bca protein assay kit catalog numbers a53225, a53226, and a53227 doc. Thermo fisher scientific, life technologies, pierce, gibco, applied biosystems, affymetrix, ebioscience, caltag, zymed, dynal, molecular probes 3,079 citations have been found for this product all reactants bos taurus bovine drosophila melanogaster fruit fly gallus gallus domesticus chicken homo sapiens human lama glama llama mus. Thermo scientific pierce bca protein assay kit reducing. Convenient1 ml ampules universalrecognized as the industry standard for protein qu.
Kit components are sufficient to complete 500 standardsize reactions 50 l protein sample plus 1 ml reagent or 2500 microscale reactions 25 l protein sample plus 200. The thermo scientific pierce bca protein assay kit. The bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Separate booklets for nucleic acid and direct a280 protein measurement methods are. Rapid gold bca protein assay kit is twocomponent, highprecision, detergentcompatible assay optimized to measure at 480nm total protein concentration compared to protein standard curve of known concentrations. Thermo scientific pierce cell lysis technical handbook. If you do not find the exact resolution you are looking for, then go for a native or higher resolution. Description the pierce micro bca protein assay kit is a threecomponent version of our bca reagents, optimized to measure total protein concentration of dilute protein solutions 0. For more information, or to download product instructions, visit. Bca assay high levels of ionic detergents require the addition of the ionic detergent compatibility reagent idcr. Pierce bovine serum albumin standard prediluted set. Add the bsa to a 100 ml volumetric flask then gradually add 1 %.
Thermo scientific pierce rapid gold bca protein assay kit is twocomponent, highprecision, detergentcompatible assay optimized to measure at 480nm total protein concentration compared to protein standard curve of known concentrations. Mixing together the three micro bca reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. Dilution scheme for standard test tube protocol and microplate procedure. Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bca based protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca, reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. Introduction the modulus by turner biosystems and the bca protein assay kit from pierce provides a simple method for quantifying protein over a large range.
Protocol the following are instructions for the microplate assay format of the pierce bca protein assay kit thermo scientific cat. Pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible protein assay for determination of protein concentration. Thermo scientific pierce pierce rapid gold bca protein. Researchers look to us for a range of innovative, highquality thermo scientific products, including our pierce antibody collection, supersignal chemiluminescent substrates, bca and other protein assay and analysis reagents, slidealyzer dialysis cassettes, cell lysis reagents, mass spectrometry sample prep and quantitation reagents, western blotting reagents and instruments, crosslinking and. Unknown samples were prepared from a dilution series of bsa at 1. Piercea protein assay kit bc thermo fisher scientific. Thermo scientific pierce bsa protein assay standards are highquality reference samples for generating accurate standard curves and calibration controls in total protein assays. Compatibility of pierce bca protein assay with promega. Thermo scientificpierce bca protein assay kit bca protein. The kit includes coomassie protein assay reagent and a package of albumin standard ampules.
Provides high accuracy results in fraction of time. Pierce bca protein assay kit university of arizona. The procedure is accomplished in less than 10 minutes with just a few simple steps. All of the lysis buffers and reagents were tested at various dilutions, ranging from 1x to 0. Gal, cat, kinase assays, western blot, elisas, immobilized glutathione pierce bca assay dilute 1. Bca reagent b, 25 ml, containing 4% cupric sulfate. Thermo scientific pierce protein assay technical handbook. Thermo scientific compatable bca protein assay kit kit with. Pierce bca protein assay kit, sufficient reagents for 250 testtube or 2500 microplate assays. The thermo scientific pierce bca protein assay kit is a detergent compatible formulation based on bicinchoninic acid bca for the. Thermo scientific pierce bca protein assay kit reducing agent compatible tube assay kit. Colorimetric method read at 562 nm linear working range for bsa is 0. Pierce bca protein assay kit brigham young university. Bca protein assay kit the bca protein assay is a simple and.
Please refer to the modelspecific user manual for more detailed instrument and software feature. Pierce protein biology thermo fisher scientific us. Download this image for free in hd resolution the choice download button below. Bca working reagent until it is light green in color. The pierce bca assay can be used to assess yields in whole cell lysates and affinitycolumn fractions, as well as to monitor protein contamination in industrial applications. Reducing agent compatible enables quantitation of total protein in samples. Bsa was diluted with distilled water to produce standards of the following concentrations. Pierce micro bca protein assay kit and reagents, thermo. Reducing agent compatible, sufficient reagents to assay 250 samples or standards of 25l each assay working range. The thermo scientific pierce bca protein assay kit is a.
Compatibility of pierce bca protein assay with promega lysis. We purchased the pierce bca protein assay kit thermo scientific with bsa standards. Microplate kit also includes assay compatible 96well microplates this products resides on a fisher scientific gsa or va contract. Bca reagent a, 250ml, contains sodium carbonate, sodium bicarbonate, bicinchoninic acid and sodium tartarate in 0. Add the bsa to a 100 ml volumetric flask then gradually add 1%. The method was automated separately on biomek i5 span8, biomek i5 multichannel and biomek i7 hybrid with spectramax i3 molecular devices. The bca protein assay kit from pierce provides the list of chemicals that have compatibility with this assay and provide an easy methodology how to interpret the results of the assay.
Number description 23225 bca protein assay kit, sufficient reagents for 500 test tube or 5,000 microplate assays. Convenientcomplete set containing seven readytouse dilutions universalrecognized. Pierce rapid gold bca protein assay kit user guide. Bicinchoninic acid protein assay kit bca1 technical bulletin. Pierce bca protein assay kit is a twocomponent, highprecision. The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the. Pierce bca reagents provide accurate determination of protein concentration with most sample types encountered in protein research. Pierce bca protein assay kit pierce bca protein assay is free hd wallpaper was upload by admin. The pierce bca protein assay is one of the most commonly used protein quantitation assays, so we have determined the compatibility of this assay with each of the lysis buffers or the reagents containing a lytic agent used in promega reporter assays. The bca assay was performed using the pierce bca protein assay kit thermo scientific. To determine the total protein concentration of a sample a bsa standard curve has to be set up with either.
Pierce microplate bca protein assay kit fisher scientific. Thermo scientific pierce bca protein assay kit reducing agent compatible. The pierce coomassie bradford protein assay kit is a readytouse, stable formulation of the traditional bradford assay reagent for measurement of total protein concentration compared to a protein standard. The thermo scientific pierce rapid gold bca protein assay kit is a rapid protein assay that uses the same copperchelating technology as the standard. Thermo scientific pierce bca protein assay kit bca. Using the matrix platemate 2x2 to automate the bca protein assay. Micro bca protein assay kit thermo fisher scientific. Measuring protein concentration using the bca protein assay. We found that the helpful point by means of using the bca protein assay kit pierce is the compatibility with more bufferssalts solvents, and detergents. Reducing agentcompatible version of our popular bca reagent kit to measure protein concentration in samples containing dtt or bme thiolreductants.
An adaptation of pierces bca protein assay reagent kit product no. The pierce micro bca protein assay reagent kit is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. For the 96 well plate assay, 8 parts of the bca working reagent are mixed with 1 part of a protein sample. Thermo scientific pierce protein assay technical handbook version 2. Measuring protein concentration using the bca protein. Open the vial containing pr1 and use the applicator provided in the kit to remove a pinhead sized amount of the compound.
Figures 35, shows the low cvs of the standards, indicating consistent sample preparation across. Colorimetric assays such as bca, pierce 660 nm, bradford, and lowry require standard curves and are more commonly used for uncharacterized protein solutions and cell lysates. The compatable preparation set is available individually or conveniently bundled with either the thermo scientific pierce bca protein assay kit or the thermo scientific pierce coomassie plus protein assay kit. Pierce 660 nm protein assay 10 bca bicinchoninic acid protein assay 11 bradford protein assay 12. Pierce bca protein assay kit reducing agent compatible. Kit type protein quantitation assay type quantitative detection method colorimetric view product on suppliers website request a quote from thermo scientific. Description pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible protein assay for determination of protein concentration. The micro bca protein assay kit is a specialized version of the popular pierce bca protein assay for determining the protein concentration of dilute samples. Thermo scientific piercepierce rapid gold bca protein assay. The procedure described in these instructions uses a simpler. Prepare pierce bovine serum albumin bsa standards 2,000. Thermo scientific pierce protein assay technical handbook nugi. Pierce bca protein assay kit thermo fisher scientific.
Tper reagent 78510, 500 ml heart, liver, kidney and brain yes luciferase. The novagen bca protein assay kit can be used to determine protein concentration in the range of 202000 gml in either a standard assay or microassay configuration. Pierce bovine serum albumin standard ampules, 2 mgml. Compare all available bca protein assays features of the micro bca protein assay kit include. The bca protein assay reducing agent compatible standard protocol. Pierce quantitative colorimetric peptide assay, 500 assays. Thermo fisher scientific, life technologies, pierce, gibco, applied biosystems, affymetrix, ebioscience, caltag, zymed, dynal, molecular probes 1,782 citations have been found for this product all reactants bos taurus bovine drosophila melanogaster fruit fly homo sapiens human locusta migratoria migratory locust macaca mulatta rhesus. Thermo scientific compatable bca protein assay kit kit. If you are not satisfied with the performance of a pierce product, please contact pierce or your local distributor. This assay exhibits linearity when using a working concentration range within 0. Bicinchoninic acid protein assay kit bca1 technical. Bca protein assay kit, sufficient reagents for 250 test tube or 2,500 microplate assays.
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